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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Jak3 Antibody #3775

Filter:
  • WB
Western Blotting Image 1: Jak3 Antibody
Western blot analysis of extracts from Jurkat cells, using Jak3 Antibody.

To Purchase # 3775

Cat. # Size Qty. Price
3775T 20 µl
$131
3775S 100 µl
$306

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 115
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Jak3 Antibody detects endogenous levels of total Jak3 protein. No cross-reactivity was detected with other family members at physiological conditions.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy-terminus of Jak3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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