Jak3 (D7B12) Rabbit mAb #8863
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 115 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Jak3 is primarily expressed in hematopoietic cells and is required for immune cell function and development (6-8). It binds to the common γ subunit (γc), a shared receptor subunit also used by several cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15 (9). IL-2 signaling and Stat5 activation are highly impaired by the loss of Jak3 (10,11). Jak3 is phosphorylated at multiple sites, including Tyr980 and 981 within the activation loop (12-14).
- Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-5.
- VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
- Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
- Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
- Thomis, D.C. et al. (1995) Science 270, 794-7.
- Nosaka, T. et al. (1995) Science 270, 800-2.
- Park, S.Y. et al. (1995) Immunity 3, 771-82.
- Russell, S.M. et al. (1994) Science 266, 1042-5.
- Johnston, J.A. et al. (1994) Nature 370, 151-3.
- Oakes, S.A. et al. (1996) Immunity 5, 605-15.
- Zhou, Y.J. et al. (1997) Proc Natl Acad Sci USA 94, 13850-5.
- Cheng, H. et al. (2008) Mol Cell Biol 28, 2271-82.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
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