JunB (C37F9) Rabbit mAb #3753
- WB
- IP
- IHC
- IF
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42, 43 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Chromatin IP | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #17148.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
- Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
- Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
- Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
- Chiu, R. et al. (1989) Cell 59, 979-986.
- Schütte, J. et al. (1989) Cell 59, 987-997.
- Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
- Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
- Passegué, E. et al. (2004) Cell 119, 431-443.
Limited Uses
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