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XML generation date: 2024-09-20 06:22:22.687
Product last modified at: 2024-05-30T07:04:02.548Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. K-Ras Antibody

K-Ras Antibody #53270

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Product Name

91054 Ras (E4K9L) Rabbit mAb
Filter:
  • WB
  • IF
This product is discontinued

Inquiry Info. # 53270

Please see our recommended alternatives.

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 21
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    K-Ras Antibody recognizes endogenous levels of total K-Ras protein. This antibody cross-reacts with N-Ras in a K-Ras preferred manner, but does not cross-react with H-Ras or R-Ras protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding V125 of human K-Ras protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

    Database Links

    UniProt ID: P01116


    Entrez-Gene Id: 3845


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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