Render Target: STATIC
Render Timestamp: 2025-01-03T12:15:59.290Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-09-20 06:19:29.090
Product last modified at: 2025-01-01T09:00:43.757Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

KCTD5 Antibody #94396

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 26
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    KCTD5 Antibody recognizes endogenous levels of total KCTD5 protein. In some cell lines, this antibody also recognizes a 100 kDa band of unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg38 of human KCTD5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    BTB/POZ domain-containing protein KCTD5 is a member of the potassium channel tetramerization domain family of proteins that play a role in transcriptional repression, cytoskeletal regulation, and ion channel gating (1). KCTD5 interacts with bound ubiquitin proteins and cullin3, and acts as a dependent E3 ligase substrate adaptor through interactions with its BTB domain (2). KCTD5 has been identified as a negative regulator of the AKT pathway by acting as an off switch for G-protein coupled receptor signaling by triggering proteolysis of Gβγ heterodimers dissociated from the Gα subunit (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.