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Render Timestamp: 2024-12-26T10:54:34.710Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:44.442
Product last modified at: 2024-09-30T08:02:05.770Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. KIR2DL2/KIR2DL3 (E7Y1V) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

KIR2DL2/KIR2DL3 (E7Y1V) Rabbit mAb #23183

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 55-70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    KIR2DL2/KIR2DL3 (E7Y1V) Rabbit mAb recognizes endogenous levels of total KIR2DL2 and KIR2DL3 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human KIR2DL2 protein.

    Background

    Killer cell immunoglobulin-like receptors (KIRs) are type 1 transmembrane glycoproteins expressed by natural killer (NK) cells and subsets of CD4, CD8, and γδ T cells (1-5). Analogous to the diversity of their human leukocyte antigen class I (HLA class I) ligands, the KIR genes are polymorphic and the content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (6,7). The KIR proteins are characterized by the number of extracellular immunoglobulin-superfamily domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain (8-10). KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM) (10), while KIR proteins with the short cytoplasmic domain lack an ITIM and instead transduce activating signals (11,12). KIR proteins play an important role in the regulation of the immune response. Combinations of KIR and HLA class I variants influence susceptibility to autoimmunity and infectious disease, as well as outcomes of haematopoietic stem cell transplantation (12-14).

    KIR2DL2 and KIR2DL3, also referred to as CD158b1 and CD158b2, respectively, regulate NK cells by interacting with the human leukocyte antigen-C1 (HLA-C1) group of molecules (15). Upon receptor ligand interaction, KIR2DL2 and KIR2DL3 inhibit the activity of NK cells thus preventing target cell lysis (16-18).
    1. Young, N.T. et al. (2001) J Immunol 166, 3933-41.
    2. Battistini, L. et al. (1997) J Immunol 159, 3723-30.
    3. Björkström, N.K. et al. (2012) Blood 120, 3455-65.
    4. Remtoula, N. et al. (2008) J Immunol 180, 2767-71.
    5. Béziat, V. et al. (2017) Immunology 150, 248-264.
    6. Uhrberg, M. et al. (1997) Immunity 7, 753-63.
    7. Shilling, H.G. et al. (2002) J Immunol 168, 2307-15.
    8. Fan, Q.R. et al. (2001) Nat Immunol 2, 452-60.
    9. Boyington, J.C. et al. (2000) Nature 405, 537-43.
    10. Vivian, J.P. et al. (2011) Nature 479, 401-5.
    11. Stewart, C.A. et al. (2005) Proc Natl Acad Sci USA 102, 13224-9.
    12. Ivarsson, M.A. et al. (2014) Front Immunol 5, 184.
    13. Kulkarni, S. et al. (2008) Semin Immunol 20, 343-52.
    14. Martin, M.P. and Carrington, M. (2013) Immunol Rev 254, 245-64.
    15. Moradi, S. et al. (2021) Nat Commun 12, 2173.
    16. Colonna, M. et al. (1993) Proc Natl Acad Sci USA 90, 12000-4.
    17. Winter, C.C. et al. (1998) J Immunol 161, 571-7.
    18. Moesta, A.K. et al. (2008) J Immunol 180, 3969-79.

    Database Links

    UniProt ID: P43627


    Entrez-Gene Id: 3803


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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