Render Target: STATIC
Render Timestamp: 2024-11-14T10:08:02.173Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:53:55.405
Product last modified at: 2024-09-30T08:02:43.313Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

KISS1R (D9D7C) Rabbit mAb #13776

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 40-140
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    KISS1R (D9D7C) Rabbit mAb recognizes endogenous levels of total KISS1R protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KISS1R protein.

    Background

    The KiSS-1 receptor (KISS1R, GPR54) is a G protein-coupled receptor that inhibits cancer cell metastasis and plays a major role in gonadotropic axis physiology (1). The GPR54 protein was originally described as an orphan receptor homologous to the galanin receptor, and later identified as a receptor for amidated peptide products of the metastasis suppressor gene KiSS-1 (KISS1, Kisspeptin-1) (2,3). In humans, amidated kisspeptin ligands are produced predominantly in cells of the arcuate nucleus and preoptic area, with expression controlled by gonadal hormones (4). Research studies show that deletion of either the KiSS-1 receptor or KiSS-1 gene leads to hypogonadotropic hypogonadism, a disorder characterized by reduced levels of circulating testosterone and gonadotropins, as well as abnormal sexual maturation (5,6). The administration of kisspeptins potently stimulates gonadotropin secretion, indicating that KISS1R and kisspeptins play a major role in the physiology of the gonadotropic axis (7). Additional research demonstrates that KISS1R and kisspeptins inhibit metastasis in cancer cells by inhibiting cell motility (8). However, other studies indicate that increased expression of KISS1R and its ligands in human breast tumors correlates with higher tumor grade and metastatic potential, likely by engaging MMP-9 activation via transactivation of EGFR (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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