Render Target: STATIC
Render Timestamp: 2024-10-17T10:52:46.344Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:58:46.304
Product last modified at: 2024-09-30T08:02:28.440Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

KLF5 (E5K6H) Rabbit mAb #40674

Filter:
  • WB
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    KLF5 (E5K6H) Rabbit mAb recognizes endogenous levels of total KLF5 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu140 of human KLF5 protein.

    Background

    Kruppel-like factors (KLFs) are a family of zinc finger DNA-binding proteins that share homology to Kruppel transcription factor in Drosophila melanogaster (1). These transcription regulators play diverse roles in cell proliferation, differentiation, inflammation, and pluripotency (2). Various KLFs, including KLF5, are involved in cancer-related processes, such as epithelial-mesenchymal transition (EMT), invasion, and metastasis (3-6). In addition, studies show that KLF5 deficiency specific to renal collecting duct epithelial cells impairs cardiac stress adaptation response coordinated by the heart and kidney (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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