Render Target: STATIC
Render Timestamp: 2024-12-23T11:01:16.638Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-02 19:16:11.045
Product last modified at: 2024-12-12T16:15:09.849Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Lamin A (133A2) Mouse mAb #86846

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 74
    Source/Isotype Mouse IgG3
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:500 - 1:2000
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:3200

    Storage

    Supplied at 1 mg/mL in PBS containing 0.09% sodium azide. Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance. This product is stable for 36 months when stored at -20C.

    Protocol

    Specificity / Sensitivity

    Lamin A (133A2) Mouse mAb recognizes endogenous levels of total lamin A protein. It also reacts with large fragments of lamin A (50 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamin C.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human lamin A protein.

    Background

    Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).
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