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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Lamin A/C Antibody #2032

Filter:
  • WB
  • IHC
Western Blotting Image 1: Lamin A/C Antibody
Western blot analysis of extracts from control HeLa cells (lane 1) or HeLa cells with an apparent in-frame truncation mutation in the gene encoding LMNA (lane 2) using Lamin A/C Antibody #2032 (upper) or β-actin (D6A8) Rabbit mAb #8457 (lower). The change in LMNA molecular weight in the mutated HeLa cells is consistent with an in-frame deletion.

To Purchase # 2032

Cat. # Size Qty. Price Ships
2032T 20 µl
$131 Jan 8
2032S 100 µl
$306 Jan 8

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 28, 70
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:300 - 1:1200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Lamin A/C Antibody detects endogenous levels of total full length lamin A (and lamin C) (70 kDa), as well as the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 (28 kDa).

Species Reactivity:

Human, Mouse, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp230 of human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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