LAMP2 (E6A6S) Rabbit mAb #34141
- WB
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100-130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:1600 - 1:3200 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 - 1:6400 |
Storage
Protocol
Specificity / Sensitivity
LAMP2 (E6A6S) Rabbit mAb recognizes endogenous levels of total LAMP2 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse LAMP2 protein.
Background
Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6).
- Eskelinen, E.L. et al. (2003) Trends Cell Biol 13, 137-45.
- Fukuda, M. (1991) J Biol Chem 266, 21327-30.
- Kundra, R. and Kornfeld, S. (1999) J Biol Chem 274, 31039-46.
- Rohrer, J. et al. (1996) J Cell Biol 132, 565-76.
- Huynh, K.K. et al. (2007) EMBO J 26, 313-24.
- Eskelinen, E.L. et al. (2004) Mol Biol Cell 15, 3132-45.
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