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Lck (L22B1) Mouse mAb

Lck (L22B1) Mouse mAb #2657

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	56
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Lck (L22B1) Mouse mAb detects endogenous levels of total Lck protein. This antibody does not cross-react with other Src family members.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-Lck fusion protein containing amino acid residues 1 to 234 of human Lck.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Database Links

UniProt ID: P06239

Entrez-Gene Id: 3932

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For Research Use Only. Not For Use In Diagnostic Procedures.

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