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XML generation date: 2025-03-07 13:10:32.483
Product last modified at: 2025-02-17T20:45:09.294Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

LDB1 Antibody #55476

Filter:
  • WB
  • IP
Western Blotting Image 1: LDB1 Antibody
Western blot analysis of extracts from various cell lines using LDB1 Antibody.

To Purchase # 55476

Cat. # Size Qty. Price
55476S 100 µl
$306

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 49
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

LDB1 Antibody recognizes endogenous levels of total LDB1 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LDB1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

LIM-domain binding protein 1 (LDB1) is a nuclear adaptor protein and transcription co-factor that interacts with a multitude of LIM-domain containing transcription factors. Through such interactions, LDB1 facilitates promoter-enhancer bridging and is necessary for the activation and/or repression of genes in multiple cell lineages, including neuronal, cardiac, and hematopoietic cell lineages. Specifically, LDB1 associates with the T cell acute lymphocytic leukemia protein 1 (TAL1) and the nuclear adaptor LIM domain only 2 (LMO2) protein to regulate erythroid gene expression (1). LDB1 is crucial for hematopoietic development, and deletion of LDB1 results in embryonic lethality (2). LDB1 is also required for the maintenance of basal mammary epithelial stem cells and promotes breast tumorigenesis (3). In addition, LDB1 stabilizes LMO2 and is necessary to promote oncogenic properties of LMO2-driven leukemia (4). Structurally, LDB1 contains an amino-terminal homodimerization domain and a carboxy-terminal LIM interaction domain (LID). This protein has no known enzymatic or nucleic-acid binding functions (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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