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Render Timestamp: 2024-11-15T10:54:40.821Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:17:33.556
Product last modified at: 2024-11-02T14:15:07.742Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. LDB1 Antibody

LDB1 Antibody #55476

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 49
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LDB1 Antibody recognizes endogenous levels of total LDB1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LDB1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    LIM-domain binding protein 1 (LDB1) is a nuclear adaptor protein and transcription co-factor that interacts with a multitude of LIM-domain containing transcription factors. Through such interactions, LDB1 facilitates promoter-enhancer bridging and is necessary for the activation and/or repression of genes in multiple cell lineages, including neuronal, cardiac, and hematopoietic cell lineages. Specifically, LDB1 associates with the T cell acute lymphocytic leukemia protein 1 (TAL1) and the nuclear adaptor LIM domain only 2 (LMO2) protein to regulate erythroid gene expression (1). LDB1 is crucial for hematopoietic development, and deletion of LDB1 results in embryonic lethality (2). LDB1 is also required for the maintenance of basal mammary epithelial stem cells and promotes breast tumorigenesis (3). In addition, LDB1 stabilizes LMO2 and is necessary to promote oncogenic properties of LMO2-driven leukemia (4). Structurally, LDB1 contains an amino-terminal homodimerization domain and a carboxy-terminal LIM interaction domain (LID). This protein has no known enzymatic or nucleic-acid binding functions (5).

    Database Links

    UniProt ID: Q86U70


    Entrez-Gene Id: 8861


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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