Render Target: STATIC
Render Timestamp:
4/1/2025, 7:34:45 AM EDT
4/1/2025, 11:34:45 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-25 22:05:23.928
Product last modified at: 2025-03-27T08:00:13.971Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LHPP (F4I8S) Rabbit mAb #31407

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 30
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LHPP (F4I8S) Rabbit mAb recognizes endogenous levels of total LHPP protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu257 of human LHPP protein.

    Background

    Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) is a highly evolutionarily conserved histidine phosphatase (1). The enzyme hydrolyzes oxygen-phosphorus bonds in inorganic pyrophosphate and nitrogen-phosphorus bonds in phospholysine, phosphohistidine, and imidodiphosphate (2). LHPP expression is downregulated in multiple cancer types (3). The protein acts as a tumor suppressor, inhibiting proliferation, migration, and invasion and promoting apoptosis during cancer development (4), probably through its inhibitory effect on Akt and TGF-β/SMAD pathway signaling (5-8). The LHPP gene has been linked to major depressive disorder (MDD), also known as clinical depression (9). In the lysosome, LHPP mediates inorganic pyrophosphate (PPi) hydrolysis (i.e., PPi to Pi) to facilitate vacuolar ATPase (V-ATPase)-dependent proton transport across the lysosomal membrane. Under stress conditions, LHPP activity maintains an acidic pH environment in the lysosome to promote C/EBPβ degradation, leading to reduced cytokine release and resulting in neurodepression (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.