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Render Timestamp: 2024-12-26T11:47:22.226Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:28.815
Product last modified at: 2024-09-30T08:01:22.928Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. LIME1 (E3M3B) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LIME1 (E3M3B) Rabbit mAb #72099

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LIME1 (E3M3B) Rabbit mAb recognizes endogenous levels of total LIME1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro211 of human LIME1 protein.

    Background

    Lck-interacting transmembrane adaptor 1 (LIME1) is a type III transmembrane adaptor protein that participates in lymphocyte signaling and is predominantly expressed in T lineage cells and plasma cells (1,2). LIME1 also harbors a palmitoylation motif for targeting to lipid rafts and potential tyrosine phosphorylation sites that are engaged upon CD4 and CD8 cross-linking (1). Research studies have demonstrated that in response to T cell receptor (TCR) engagement, LIME1 expression is upregulated, undergoes tyrosine phosphorylation, and associates with numerous signaling proteins such as Lck, GADS, and Vav. Collectively, these observations point toward a role for LIME1 in the regulation of T cell activation and adhesion (3,4).

    Database Links

    UniProt ID: Q9H400


    Entrez-Gene Id: 54923


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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