Render Target: STATIC
Render Timestamp: 2024-11-14T09:43:06.294Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:58:07.872
Product last modified at: 2024-10-25T16:00:11.689Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LIMP-2/SCARB2 (E2Z5F) Rabbit mAb #27960

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 54, 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #69463.

    Protocol

    Specificity / Sensitivity

    LIMP-2/SCARB2 (E2Z5F) Rabbit mAb recognizes endogenous levels of total LIMP-2/SCARB2 protein. LIMP-2/SCARB2 is glycosylated at multiple sites, and it migrates at 80 kDa and 54 kDa in its glycosylated and unglycosylated states, respectively.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly467 of mouse LIMP-2/SCARB2 protein.

    Background

    Lysosomal integral membrane protein type 2 (LIMP-2, also known as SCARB2) is a lysosomal membrane glucoprotein encoded by the SCARB2 gene. As a lysosome-enriched protein,  LIMP-2/SCARB2 contributes to endosomal and lysosomal transport and function in the cell. β-glucocerebrosidase (GBA), a lysosomal enzyme defective in Gaucher disease and genetically linked to Parkinson’s disease, is targeted to lysosomes by binding directly to LIMP-2/SCARB2 via a mannose 6-phosphate-independent pathway (1-3). LIMP-2/SCARB2 may also play a role in the transport of cholesterol from the lysosome to the membrane, an important function as cholesterol contributes to various biophysical properties of cell membranes and may contribute to various diseases (4,5). Interestingly, SCARB2 is upregulated in neurodegenerative diseases, including Lewy body disease (6), and genetic variants in the gene encoding SCARB2 are linked to Parkinson’s disease (7), suggesting that altered lysosomal function may contribute to neuropathology in these diseases. LIMP-2/SCARB2 also serves as a receptor for enterovirus 71 and coxsackieviruses (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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