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Render Timestamp: 2024-11-22T12:09:23.361Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:18.849
Product last modified at: 2024-11-14T13:45:08.374Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. LTβR (E5I6X) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LTβR (E5I6X) Rabbit mAb #57560

Filter:
  • WB
  • IP
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45-70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LTβR (E5I6X) Rabbit mAb recognizes endogenous levels of total LTβR protein. This antibody is expected to recognize multiple isoforms of LTβR protein. This antibody cross-reacts with an unidentified protein of 20 kDa and 30 kDa in some cell extracts.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg428 of human LTβR protein.

    Background

    Lymphotoxin β receptor (LTβR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3) and tumor necrosis factor receptor type III (TNF-RIII), is a type I membrane receptor expressed by stromal cells, including endothelial, mesenchymal, and epithelial cells, and by myeloid cells, such as dendritic cells and macrophages (1-4). When lymphotoxin α (LTα) is co-expressed with lymphotoxin β (LTβ) that possesses a transmembrane domain, it forms a cell surface-bound heterotrimer (LTα1β2) that exclusively binds to LTβR (5,6). The LTα1β2/LTβR pathway is required for the normal development of lymph nodes and Peyer’s patches (7,8). Disruption of the LTα1β2/LTβR pathway results in impaired protection from viral and bacterial infections (9,10). LTβR is also a receptor for LIGHT/TNFSF14 (11,12). LTβR signaling through LIGHT/TNFSF14 regulates self-renewal and differentiation of hematopoietic and leukemic stem cells (13). In cancer cells, LTβR signaling via TRAF molecules promotes apoptosis (14-16). Agonistic antibodies to LTβR trigger cancer cell death and suppress tumor growth in vivo (17).
    1. Murphy, M. et al. (1998) Cell Death Differ 5, 497-505.
    2. Browning, J.L. and French, L.E. (2002) J Immunol 168, 5079-87.
    3. Browning, J.L. et al. (1997) J Immunol 159, 3288-98.
    4. Kabashima, K. et al. (2005) Immunity 22, 439-50.
    5. Androlewicz, M.J. et al. (1992) J Biol Chem 267, 2542-7.
    6. Browning, J.L. et al. (1993) Cell 72, 847-56.
    7. De Togni, P. et al. (1994) Science 264, 703-7.
    8. Fütterer, A. et al. (1998) Immunity 9, 59-70.
    9. Ehlers, S. et al. (2003) J Immunol 170, 5210-8.
    10. Wege, A.K. et al. (2014) Innate Immun 20, 461-70.
    11. Maeda, T. et al. (2018) J Immunol 201, 202-214.
    12. Schneider, K. et al. (2004) Immunol Rev 202, 49-66.
    13. Höpner, S.S. et al. (2021) Nat Commun 12, 1065.
    14. Browning, J.L. et al. (1996) J Exp Med 183, 867-78.
    15. Force, W.R. et al. (1997) J Biol Chem 272, 30835-40.
    16. Rooney, I.A. et al. (2000) J Biol Chem 275, 14307-15.
    17. Lukashev, M. et al. (2006) Cancer Res 66, 9617-24.

    Database Links

    UniProt ID: P36941


    Entrez-Gene Id: 4055


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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