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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Ly-6G (E6Z1T) Rabbit mAb #87048

Filter:
  • WB
  • IHC
Western Blotting Image 1: Ly-6G (E6Z1T) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length mouse Ly-6C1 protein (mLy-6C1-Myc/DDK), Myc/DDK-tagged full-length mouse Ly-6C2 protein (mLy-6C2-Myc/DDK), Myc/DDK-tagged full-length mouse Ly-6F protein (mLy-6F-Myc/DDK), Myc/DDK-tagged full-length mouse Ly-6I protein (mLy-6I-Myc/DDK), and Myc/DDK-tagged full-length mouse Ly-6G protein (mLy-6G-Myc/DDK), using Ly-6G (E6Z1T) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 87048

Cat. # Size Qty. Price
87048T 20 µl
$153
87048S 100 µl
$357

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 20-40
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:300 - 1:1200
Immunohistochemistry (Paraffin) 1:75 - 1:300

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #75028.

Protocol

Specificity / Sensitivity

Ly-6G (E6Z1T) Rabbit mAb recognizes endogenous levels of total Ly-6G protein. This antibody does not cross-react with Ly-6C1, Ly-6C2, Ly-6F, or Ly-6I. Non-specific staining was observed in mouse gastrointestinal epithelium by immunohistochemistry.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse Ly-6G recombinant protein.

Background

The Ly-6 complex is a series of genes found on chromosome 15. These genes code for a number of different proteins that can be used as surface markers. The family members vary in their biologic expression and have been shown to be involved in cell signaling and cell adhesion (1). The structure of these proteins includes a motif known as the LU domain that has three loops comprised of disulfide bonds. These bonds are formed by 8 to 10 cysteines that can cause differences in the length of the loops as well as the sequences at each tip (2,4). There are 11 known Ly-6 genes on murine chromosome 15 that code for different proteins. These family members, excluding secreted Ly6/Plaur domain containing 1 coded by the Slurp1 gene, are attached to the cell surface by a GPI anchor near the C terminus. The structure of these proteins may play a role in transmembrane interactions, and downstream signaling cascades (1,2).

Ly-6 proteins have been widely used as differentiation markers on hematopoietic cells. The ability to isolate and express specific Ly-6 antibodies through hybridoma technology has allowed researchers to identify unique proteins (1). These proteins are expressed on subsets of immune cells at different stages of development, such as T cells, B cells, monocytes, granulocytes, and macrophages (1-5). 

Ly-6G is used as a marker for mouse neutrophils (2,3). It is also expressed by mouse polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) (6).
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