Ly-6G/Ly-6C (Gr-1) (RB6-8C5) Rat mAb #31469
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Ly-6 proteins have been widely used as differentiation markers on hematopoietic cells. The ability to isolate and express specific Ly-6 antibodies through hybridoma technology has allowed researchers to identify unique proteins (1). These proteins are expressed on subsets of immune cells at different stages of development, such as T cells, B cells, monocytes, granulocytes, and macrophages (1-5).
The 1A8 clone is specific to Ly-6G, which is used as a marker for mouse neutrophils (2,3). It is also expressed by mouse polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) (6). The RB6-8C5 clone recognized both Ly-6G and Ly-6C, also known as Gr-1, and has been found to express on neutrophils, monocytes, dendritic cells, and T cells (2,3).
Limited Uses
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