Lysyl Hydroxylase 2 Antibody #44709
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Collagen content and abundance determines the nature of the scaffold of the ECM, and is known to influence pathological events such as tumor growth and invasion, and also tissue fibrosis (1). Lysyl Hydroxylase 2 (encoded by the PLOD2 gene), is an important enzyme that mediates the hydroxylation of lysines in collagen-like peptides in the ER, a key step that contributes to cross-linking of collagen fibrils (4). Interestingly, overexpression of Lysyl Hydroxylase 2 observed in multiple cancers, driven potentially through hypoxia-induced mechanisms, is related to a poor prognosis, tumor migration, invasion, and EMT (5,6). Current interest exists in pharmaceutical inhibition of this enzyme for therapeutic intervention to prevent metastasis and tumor spread (6).
- Barkan, D. et al. (2010) Eur J Cancer 46, 1181-8.
- Hynes, R.O. (2009) Science 326, 1216-9.
- Ricard-Blum, S. (2011) Cold Spring Harb Perspect Biol 3, a004978.
- van der Slot, A.J. et al. (2003) J Biol Chem 278, 40967-72.
- Song, Y. et al. (2017) Oncotarget 8, 41947-41962.
- Du, H. et al. (2017) Biomed Pharmacother 90, 670-676.
Limited Uses
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