Render Target: STATIC
Render Timestamp: 2024-11-29T12:19:37.501Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-08-01 15:27:54.074
Product last modified at: 2024-05-30T07:14:08.203Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

M-Cadherin Antibody #45863

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Inquiry Info. # 45863

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    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 130
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    M-cadherin recognizes endogenous levels of total M-cadherin protein. This antibody also cross-reacts with a number of unidentified proteins between 40 kDa and 95 kDa.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg434 of mouse M-cadherin protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).
    M-cadherin is a cell-cell adhesion molecule expressed in satellite cells, a collection of adult stem cells/myogenic progenitors found in mature muscle tissue (9). Research studies indicate that M-cadherin may be involved in the recognition and fusion of adjacent cells, and may play an important role in activating satellite cell division (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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