Render Target: STATIC
Render Timestamp: 2024-09-26T10:05:06.816Z
Commit: 60a5021c3a47fc24d1656fb463e2c3c41a1ad145
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MAdCAM-1 (E1V8F) Rabbit mAb #93757

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous (IHC-P), Transfected (W)
    MW (kDa) 60-85
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #41968.

    Protocol

    Specificity / Sensitivity

    MAdCAM-1 (E1V8F) Rabbit mAb recognizes transfected levels of total MAdCAM-1 protein by western blot and endogenous levels of total MAdCAM-1 protein by immunohistochemistry. The observed molecular weight of 60-85 kDa differs from the predicted molecular weight of 40 kDa due to glycosylation.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAdCAM-1 protein.

    Background

    Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is a glycoprotein expressed by endothelial cells at venules of Peyer's patches, intestinal lamina propria, and mesenteric lymph nodes (1). MAdCAM-1 contributes to lymphocyte homing to these tissues, serving as a ligand for α4β7, L-Selectin, and, to a lesser extent, α4β1 (2-6). Expression of MAdCAM-1 is upregulated in patients with inflammatory bowel disease, and blocking MAdCAM-1 reduces colon inflammation by disrupting lymphocyte homing to the intestinal tract (7). In ulcerative colitis, disease activity may be a result of the L-Selectin ligand carbohydrates attached to MAdCAM-1 by N-acetylglucosamine-6-O-sulfotransferase-1 (GlcNAc6ST-1) rather than the expression levels of MAdCAM-1 alone (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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