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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MAdCAM-1 (E1V8F) Rabbit mAb #93757

Filter:
  • WB
  • IHC
Western Blotting Image 1: MAdCAM-1 (E1V8F) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length MAdCAM-1 protein (MAdCAM-1-Myc/DDK; +), using MAdCAM-1 (E1V8F) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 93757

Cat. # Size Qty. Price
93757T 20 µl
$173
93757S 100 µl
$402

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous (IHC-P), Transfected (W)
MW (kDa) 60-85
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #41968.

Protocol

Specificity / Sensitivity

MAdCAM-1 (E1V8F) Rabbit mAb recognizes transfected levels of total MAdCAM-1 protein by western blot and endogenous levels of total MAdCAM-1 protein by immunohistochemistry. The observed molecular weight of 60-85 kDa differs from the predicted molecular weight of 40 kDa due to glycosylation.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAdCAM-1 protein.

Background

Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is a glycoprotein expressed by endothelial cells at venules of Peyer's patches, intestinal lamina propria, and mesenteric lymph nodes (1). MAdCAM-1 contributes to lymphocyte homing to these tissues, serving as a ligand for α4β7, L-Selectin, and, to a lesser extent, α4β1 (2-6). Expression of MAdCAM-1 is upregulated in patients with inflammatory bowel disease, and blocking MAdCAM-1 reduces colon inflammation by disrupting lymphocyte homing to the intestinal tract (7). In ulcerative colitis, disease activity may be a result of the L-Selectin ligand carbohydrates attached to MAdCAM-1 by N-acetylglucosamine-6-O-sulfotransferase-1 (GlcNAc6ST-1) rather than the expression levels of MAdCAM-1 alone (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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