MARK2 Antibody #9118
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 78, 82 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys430 of human MARK2. Antibodies were purified by protein A and peptide affinity chromatography.
Background
Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7).
MARK2 (4), also termed as Par-1 (8) and EMK1 (9), contributes to cellular polarity, cell cycle progression, microtuble dynamics, and neurite outgrowth. The MARK2 gene encodes at least two alternatively spliced isoforms that are co-expressed in various cell lines (10). Substrates of MARK2 include microtubule associated protein (MAPs), tau, histone deacetylases (11), and Rab11-FIP2 (12). Knockout studies suggest that MARK2 plays an essential role in immune system function (13), glucose homeostasis (14), and learning and memory (15).
- Drubin, D.G. and Nelson, W.J. (1996) Cell 84, 335-44.
- Illenberger, S. et al. (1996) J Biol Chem 271, 10834-43.
- Drewes, G. et al. (1995) J Biol Chem 270, 7679-88.
- Drewes, G. et al. (1997) Cell 89, 297-308.
- Kato, T. et al. (2001) Neoplasia 3, 4-9.
- Trinczek, B. et al. (2004) J Biol Chem 279, 5915-23.
- Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
- Guo, S. and Kemphues, K.J. (1995) Cell 81, 611-620.
- Inglis, J.D. et al. (1993) Mamm. Genome 4, 401-403.
- Espinosa, L. and Navarro, E. (1998) Cytogenet. Cell Genet. 81, 278-282.
- Dequiedt, F. et al. (2006) Mol. Cell. Biol. 26, 7086-7102.
- Ducharme, N.A. et al. (2006) Mol. Biol. Cell 17, 3625-3637.
- Hurov, J.B. et al. (2001) Mol. Cell. Biol. 21, 3206-3219.
- Hurov, J.B. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 5680-5685.
- Segu, L. et al. (2008) Neurobiol. Aging 29, 231-240.
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