MAT2A (E5I7C) Rabbit mAb #84478

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	47, 49
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MAT2A (E5I7C) Rabbit mAb recognizes endogenous levels of total MAT2A protein. This antibody does not cross-react with MAT1A protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAT2A protein.

Background

Methionine adenosyltransferase II alpha (MAT2A) uses methionine and ATP as substrates to generate S-adenosylmethionine (SAM), a universal methyl group donor in cells. Tumor-initiating cells (TICs) have high methionine cycle activity mediated by MAT2A, leading to their metabolic dependency on exogenous methionine (1). Studies show that mTORC1 signaling induces MAT2A expression, leading to elevated synthesis of SAM. In addition, mTORC1 increases the protein level of Wilms' tumor 1-associating protein (WTAP), a positive regulatory subunit of the human N⁶-methyladenosine (m⁶A) RNA methyltransferase complex. mTORC1, therefore, promotes N⁶-methylation of mRNA to stimulate protein synthesis and cell growth (2). Furthermore, inhibition of MAT2A activity was shown to decrease proliferation of cancer cells deficient in methylthioadenosine phosphorylase (MTAP) (3).

Database Links

UniProt ID: P31153

Entrez-Gene Id: 4144

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For Research Use Only. Not For Use In Diagnostic Procedures.

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