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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

mCherry (E5D8F) Rabbit mAb #43590

Filter:
  • WB
  • IF
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with Myc/DDK C-terminally tagged mCherry (mCherry C-tagged) or Myc/DDK N-terminally tagged mCherry (mCherry N-tagged), using mCherry (E5D8F) Rabbit mAb (upper) and #8457 β-Actin (D6A8) Rabbit mAb (lower).

To Purchase # 43590

Cat. # Size Qty. Price
43590T 20 µl
$167
43590S 100 µl
$339

Product Specifications

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Frozen) 1:50 - 1:200
Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

mCherry (E5D8F) Rabbit mAb detects mCherry-tagged proteins (either N-terminal tagged or C-terminal tagged) exogenously expressed in cells. Please note that the mCherry tags add approximately 28kDa to the molecular weight of the fusion protein.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a full length recombinant mCherry protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

mCherry is a monomeric, fluorescent, photostable red dye that was isolated from and further engineered from the Discosoma sea anemone (1). Its peak fluorescent excitation is at 587 nm and emission 610 nm (1).
For Research Use Only. Not for Use in Diagnostic Procedures.
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