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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

MCP-1 Antibody (Carboxy-terminal Antigen) #39091

Filter:
  • WB
  • IP
Western Blotting Image 1: MCP-1 Antibody (Carboxy-terminal Antigen)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human MCP-1 protein (hMCP-1; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-actin (D6A8) Rabbit mab #8457 (lower).

To Purchase # 39091

Cat. # Size Qty. Price Ships
39091S 100 µl
$306 Jan 22

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 13-15
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MCP-1 Antibody (Carboxy-terminal Antigen) recognizes endogenous levels of total MCP-1 protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MCP-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Monocyte chemoattractant protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G protein-coupled receptor CCR2 (9).
For Research Use Only. Not For Use In Diagnostic Procedures.
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