MCRIP1 (D2Y8V) Rabbit mAb #78128
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 11 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
MCRIP1 (D2Y8V) Rabbit mAb recognizes endogenous levels of total MCRIP1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro56 of human MCRIP1 protein.
Background
Mapk-regulated corepressor-interacting protein 1 (MCRIP1) is a widely expressed protein substrate of ERK. MCRIP1 functions in regulating transcriptional repression of E-cadherin by the transcriptional corepressor CtBP. Unphosphorylated MCRIP1 binds to CtBP, preventing its transcriptional regulatory activity. When MCRIP1 is phosphorylated by ERK, CtIP is free to interact with the transcriptional repressor, ZEB1. The CtIP/ZEB1 complex then silences E-cadherin expression, promoting epithelial-mesenchymal transition (EMT), a process in tumor progression that leads to enhanced migration and invasion (1,2).
Limited Uses
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