Render Target: STATIC
Render Timestamp: 2024-11-21T12:58:22.380Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:29.214
Product last modified at: 2024-09-30T08:01:41.735Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MED13 (E4E9H) Rabbit mAb #91684

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 230
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MED13 (E4E9H) Rabbit mAb recognizes endogenous levels of total MED13 protein. This antibody recognizes a 50 kDa protein of unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1592 of human MED13 protein.

    Background

    The mammalian Mediator complex is a multi-subunit protein complex that acts to relay signals from transcription factors to RNA polymerase II (Pol II) and the basal transcription machinery. In other words, Mediator acts as a centralized hub or integrator for transcriptional regulation (reviewed in 1,2). Multiple studies have shown that Mediator functions include epigenetic regulation, transcriptional elongation, transcription termination, mRNA processing, non-coding RNA activation, and super-enhancer formation. Through these functions, Mediator contributes to the regulation of many biological processes, including insulin signaling (3), NF-κB-dependent signaling (4), stem cell pluripotency and self-renewal (5,6), and proliferation of colon cancer cells (7,8). The Mediator complex consists of 31 protein subunits and can be divided into four distinct modules: the head, middle, tail, and CDK8 kinase module. The CDK8 kinase module contains the CDK8, cyclin C, MED12, and MED13 subunits and is thought to act as a molecular switch, phosphorylating the C-terminal domain (CTD) of Rpb1, the large subunit of Pol II, to inhibit Pol II recruitment and transcription initiation (9,10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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