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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Melanotransferrin (F3W8H) Rabbit mAb #20173

Filter:
  • WB
  • IP
Western Blotting Image 1: Melanotransferrin (F3W8H) Rabbit mAb
Western blot analysis of extracts from SK-MEL-30, SK-MEL-5, and HeLa cells using Melanotransferrin (F3W8H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of melanotransferrin protein in HeLa cells is consistent with published observations.

To Purchase # 20173

Cat. # Size Qty. Price Ships
20173T 20 µl
$155
20173S 100 µl
$371

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 97
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Melanotransferrin (F3W8H) Rabbit mAb recognizes endogenous levels of total melanotransferrin protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly668 of human melanotransferrin protein.

Background

Melanotransferrin, also known as MTF, MFI2, CD228, or p97, is a membrane-bound glycoprotein that is commonly associated with melanoma (1). Melanotransferrin shares structural similarity and iron-binding properties with the protein transferrin, although it does not appear to be involved with iron transport (2-4). Melanotransferrin is expressed at low levels in normal tissue but has elevated expression in melanoma (5,6). The exact biological function of melanotransferrin in melanoma is not yet fully understood, but it is believed to play a role in cell proliferation and invasion (7). Upregulation of melanotransferrin has also been observed in other forms of cancer, such as lung, colorectal, gastric, and oral squamous cell carcinoma (OSCC) (8-11).
For Research Use Only. Not For Use In Diagnostic Procedures.
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