Render Target: STATIC
Render Timestamp: 2024-12-11T11:36:33.143Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:58:30.268
Product last modified at: 2024-09-30T08:01:33.375Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. MERS-CoV Envelope Protein (E8X6R) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MERS-CoV Envelope Protein (E8X6R) Rabbit mAb #89952

Filter:
  • WB

    Supporting Data

    REACTIVITY Vir
    SENSITIVITY Transfected Only
    MW (kDa) 12
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MERS-CoV Envelope Protein (E8X6R) Rabbit mAb recognizes transfected levels of total MERS-CoV envelope protein.

    Species Reactivity:

    Virus

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro75 of MERS-CoV envelope protein.

    Background

    Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus. Similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV genome encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to at least 10 smaller open reading frames (ORFs) that encode the non-structural (accessory) proteins involved in viral replication (3,4). The MERS envelope protein (E) is the smallest of the structural proteins, and is abundantly expressed in the host cell during viral replication, despite only a fraction of synthesized E protein ultimately being incorporated into new viral particles (5). As in other betacoronaviruses, the MERS E protein likely functions as an ion channel (viroporin), involved in transmembrane molecular transport (6). Within infected host cells, E protein is largely localized to endoplasmic reticulum, Golgi, and related intermediate structures (e.g., ERGIC), consistent with a functionally important role in assembly, budding, and intracellular trafficking of new virion particles (5). This is supported by studies showing that MERS virions were incapable of replication after deletion of the gene encoding the E protein (7).

    Database Links

    UniProt ID: K9N5R3


    Entrez-Gene Id: 37616437


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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