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XML generation date: 2024-09-30 01:58:30.184
Product last modified at: 2025-01-01T09:02:48.320Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MERS-CoV Membrane Protein (E2Z4Q) Rabbit mAb #56075

Filter:
  • WB
Western Blotting Image 1: MERS-CoV Membrane Protein (E2Z4Q) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or transiently transfected with expression constructs encoding Myc/DDK-tagged MERS-CoV membrane protein (lane 2), using MERS-CoV Membrane Protein (E2Z4Q) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 56075

Cat. # Size Qty. Price Ships
56075T 20 µl
$153
56075S 100 µl
$339

Supporting Data

REACTIVITY Vir
SENSITIVITY Transfected Only
MW (kDa) 20-30
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • Vir-Virus 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MERS-CoV Membrane Protein (E2Z4Q) Rabbit mAb recognizes transfected levels of total MERS-CoV membrane protein. This antibody does not cross-react with membrane proteins from SARS coronaviruses (CoV-1, CoV-2).

Species Reactivity:

Virus

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln184 of MERS-CoV membrane protein.

Background

Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus; similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV genome encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to at least 10 smaller open reading frames (ORFs) that encode the non-structural (accessory) proteins required for viral replication (3). The MERS membrane protein, like that of other betacoronaviruses, is an integral membrane protein and the most abundant of the structural proteins in a virus particle. Numerous studies have shown that the membrane protein of betacoronaviruses is more than just a structural protein; it plays multiple functional roles in orchestrating viral replication following host infection (4-7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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