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Product last modified at: 2025-01-01T09:10:20.831Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MERS-CoV Spike Protein (E6Q5A) Rabbit mAb #41859

Filter:
  • WB
Western Blotting Image 1: MERS-CoV Spike Protein (E6Q5A) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or transiently transfected with an expression construct encoding Myc/DDK-tagged MERS-CoV spike (lane 2), using MERS-CoV Spike Protein (E6Q5A) Rabbit mAb
(upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The antibody detects full-length (uncleaved) MERS-CoV spike protein, and the fragment corresponding to the S1 domain generated by endogenous protease cleavage.

To Purchase # 41859

Cat. # Size Qty. Price Ships
41859T 20 µl
$153
41859S 100 µl
$339

Supporting Data

REACTIVITY Vir
SENSITIVITY Transfected Only
MW (kDa) 200, 120
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • Vir-Virus 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MERS-CoV Spike Protein (E6Q5A) Rabbit mAb recognizes transfected levels of total MERS-CoV spike protein. The antibody detects full-length MERS-CoV spike protein and also detects the S1 fragment generated by host protease cleavage. It does not cross-react with spike proteins from SARS coronaviruses (CoV-1, CoV-2).

Species Reactivity:

Virus

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MERS-CoV spike protein.

Background

Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus; similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV virion is comprised of four key structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (3). Coronavirus spike proteins are class I fusion proteins that harbor an ectodomain, a transmembrane domain, and an intracellular tail. The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases (4). MERS-CoV spike proteins utilize the cell surface protein DPP4/CD26 as the receptor for cellular entry (5,6); notably, this protein displays no structural similarities to ACE2, the primary receptor for SARS-CoV-1 and SARS-CoV-2. Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (7,8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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