Methyl-Histone H3 (Lys27) Antibody Sampler Kit #53312
Product Information
Kit Usage Information
Protocols
- 4499: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 7074: Western Blotting
- 9728: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow, ChIP Magnetic
- 9733: Western Blotting, Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay, CUT&Tag
- 84932: Western Blotting, Immunoprecipitation (Magnetic)
Product Description
Specificity / Sensitivity
Source / Purification
Background
Methylation of histone H3 Lys27 is generally associated with transcriptional repression and regulation of stem cell pluripotency and differentiation, through the regulation of facultative heterochromatin. Mono-, di-, and tri-methylation of histone H3 Lys27 are all mediated by polycomb repressor complex 2 (PRC2), which contains either the EZH1 or EZH2 methyltransferase proteins. Tri- and di-methyl-histone H3 Lys27 levels are highest at the promoters of polycomb-repressed genes. In addition, tri- and di-methyl-histone H3 Lys27 is found at the promoters of inactive, but transcriptionally poised genes that also contain the active tri-methyl-histone H3 Lys4 modification. Mono-methyl-histone H3 Lys27 is more widely dispersed and found in the bodies of active genes.
- Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
- Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop, 1-27.
- Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
- Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70.
- Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26.
- Shi, X. et al. (2006) Nature 442, 96-9.
- Wysocka, J. et al. (2006) Nature 442, 86-90.
- Wysocka, J. et al. (2005) Cell 121, 859-72.
- Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.