Methyl-Histone H3 (Lys4) Antibody Sampler Kit #26482
Product Information
Kit Usage Information
Protocols
- 4499: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 5326: Western Blotting, Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay, CUT&Tag
- 7074: Western Blotting
- 9725: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay, CUT&Tag
- 9751: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay, CUT&Tag
Product Description
Specificity / Sensitivity
Source / Purification
Background
Methylation of histone H3 Lys4 is associated with transcriptional activation. Mono-methyl-histone H3 Lys4 levels are high at trancriptional enhancer elements, with lower levels of mono-methylation found at the promoters of active genes. Tri-methyl-histone H3 Lys4 levels are high at the promoters of active genes, in addition to bivalent, transcriptionally poised genes that also contain the repressive tri-methyl-histone H3 Lys27 modification. Di-methyl-histone H3 Lys4 levels are highest in the 5'-end of transcriptionally active genes.
- Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
- Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop, 1-27.
- Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
- Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70.
- Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26.
- Shi, X. et al. (2006) Nature 442, 96-9.
- Wysocka, J. et al. (2006) Nature 442, 86-90.
- Wysocka, J. et al. (2005) Cell 121, 859-72.
- Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.
Limited Uses
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