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Render Timestamp: 2024-11-22T11:08:13.664Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:24.571
Product last modified at: 2024-11-12T21:00:11.249Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. METTL16 (E2J1D) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

METTL16 (E2J1D) Rabbit mAb #87538

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 78
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    METTL16 (E2J1D) Rabbit mAb recognizes endogenous levels of total METTL16 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala9 of human METTL16 protein.

    Background

    METTL16 is an N6-adenosine methyltransferase responsible for the regulation of the MAT2A gene, which encodes S-adenosylmethionine (SAM) synthase. Upon SAM depletion, MAT2A expression increases due to a splicing event of a retained intron. Alternative splicing and mRNA stability is governed by adenosine methylation in the 3’ UTR of the MAT2A mRNA by METTL16. These marks are then read by YTHDC1, and knockdown of either METTL16 or YTHDC1 results in decreased response to lack of SAM (1,2). The METTL16 methyltransferase domain differs from METTL3 and METTL14 as it contains an extra N-terminal module, suggesting a different set of target mRNAs (3,4). Lack of METTL16 during development has been shown to be embryonically lethal, resulting in a dysregulated transcriptome that cannot proceed past the 64-cell blastocyst stage (3).

    Database Links

    UniProt ID: Q86W50


    Entrez-Gene Id: 79066


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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