Microglia Interferon-Related Module Antibody Sampler Kit #19171
Product Information
Kit Usage Information
Protocols
- 3892: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
- 4060: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 7074: Western Blotting
- 14982: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 67824: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
- 72604: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence*, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay
- 88410: Western Blotting, Immunofluorescence*, Flow
Product Description
Specificity / Sensitivity
Source / Purification
Background
Stat2 is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (5,6). In response to IFN-alpha/beta, Stat2 is activated by phosphorylation at site Tyr690 through associations with receptor-bound Jak kinases (7). Akt is a protein kinase that plays a critical role in controlling survival and apoptosis. Akt is activated by various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (8-10) and its activity is shown to be essential for up-regulation of key IFN inducible proteins (11). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (12) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (13,14).
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- Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
- Improta, T. et al. (1994) Proc Natl Acad Sci U S A 91, 4776-80.
- Franke, T.F. et al. (1997) Cell 88, 435-7.
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- Kaur, S. et al. (2008) Proc Natl Acad Sci U S A 105, 4808-13.
- Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
- Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
- Jacinto, E. et al. (2006) Cell 127, 125-37.
Limited Uses
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