Microglia Proliferation Module Antibody Sampler Kit #49938
Product Information
Kit Usage Information
Protocols
- 2623: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence
- 2808: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 2914: Western Blotting, Immunofluorescence, Flow
- 3619: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, ChIP Magnetic
- 3892: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
- 7074: Western Blotting
- 9129: Immunofluorescence, Immunofluorescence, Flow
- 67824: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Immunofluorescence, Flow
Product Description
Specificity / Sensitivity
Source / Purification
Background
Ki-67 is a nuclear nonhistone protein (5) universally expressed among proliferating cells and absent in quiescent cells (6). Minichromosome maintenance protein 2 (MCM2) is a nuclear protein that plays a role in DNA replication and cell division (7) and is commonly used as a marker for cell proliferation, including brain tissue (8). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (9). Aurora A, B, and C are a family of highly conserved serine/threonine kinases that regulate chromosomal alignment and segregation during mitosis and meiosis. Their activity requires autophosphorylation of a threonine within their kinase domain at site Thr288 of Aurora A, Thr232 of Aurora B, and Thr198 of Aurora C (10). Heterochromatin protein 1 (HP1) α and β are heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (11).
- Friedman, B.A. et al. (2018) Cell Rep 22, 832-47.
- Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
- Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
- Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.
- Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
- Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
- Mincheva, A. et al. (1994) Cytogenet Cell Genet 65, 276-7.
- Doorn, K.J. et al. (2014) Neural Plast 2014, 959154.
- Li, F. et al. (1999) Nat Cell Biol 1, 461-6.
- Goldenson, B. and Crispino, J.D. (2015) Oncogene 34, 537-45.
- Maison, C. and Almouzni, G. (2004) Nat Rev Mol Cell Biol 5, 296-304.
Limited Uses
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