Render Target: STATIC
Render Timestamp: 2024-12-20T11:04:24.596Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:57:14.018
Product last modified at: 2024-12-17T19:05:23.259Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MITF (D3B4T) Rabbit mAb #97800

Filter:
  • WB
  • IF
  • ChIP
  • C&R
  • C&T

    Supporting Data

    REACTIVITY H M Mk
    SENSITIVITY Endogenous
    MW (kDa) 50-75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • ChIP-Chromatin Immunoprecipitation 
    • C&R-CUT & RUN 
    • C&T-CUT & Tag 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:200
    Chromatin IP 1:50
    Chromatin IP-seq 1:50
    CUT&RUN 1:50
    CUT&Tag 1:50

    Protocol

    Specificity / Sensitivity

    MITF (D3B4T) Rabbit mAb recognizes endogenous levels of total MITF protein.

    Species Reactivity:

    Human, Mouse, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human MITF protein.

    Background

    Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development (1-3). In humans, MITF can function as a melanoma oncogene (4) and mutations in the corresponding MITF gene are associated with Waardenburg syndrome type 2, an auditory-pigmentary syndrome characterized by developmental defects in cells derived from neural crest (5). At least 12 isoforms of MITF have been identified, which exhibit differential patterns of expression among cell and tissue types (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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