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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
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Product last modified at: 2025-03-31T16:45:09.323Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Miwi (G82) Antibody #2079

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: Miwi (G82) Antibody
Western blot analysis of extracts from various mouse tissues using Miwi (G82) Antibody (upper panel) or β-Actin Antibody (#4967) (lower panel).

To Purchase # 2079

Cat. # Size Qty. Price
2079S 100 µl
$306

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 90
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Frozen) 1:200 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Miwi (G82) Antibody detects endogenous levels of total Miwi protein.

Species Reactivity:

Human, Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Gly82 of mouse Miwi. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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