MLLT1/ENL (D9M4B) Rabbit mAb #14893
- WB
- ChIP
- C&R
- C&T
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
- C&T-CUT & Tag
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
MLL translocated to 1/eleven-nineteen-leukemia (MLLT1/ENL) is also found as part of the histone H3 Lys79 methyltransferase disruptor of telomeric silencing-like (Dot1L) complex that has been suggested to play a role in transcription elongation. This complex regulates the expression of genes, such as the Wnt-signaling pathway target genes that control cell proliferation and differentiation during development (12,13).
- Mohan, M. et al. (2010) Nat Rev Cancer 10, 721-8.
- Lin, C. et al. (2010) Mol Cell 37, 429-37.
- Drexler, H.G. et al. (2004) Leukemia 18, 227-32.
- Smith, E. et al. (2011) Genes Dev 25, 661-72.
- Shilatifard, A. et al. (1996) Science 271, 1873-6.
- Shilatifard, A. et al. (1997) Proc Natl Acad Sci U S A 94, 3639-43.
- Miller, T. et al. (2000) J Biol Chem 275, 32052-6.
- Lin, C. et al. (2011) Genes Dev 25, 1486-98.
- Yokoyama, A. et al. (2010) Cancer Cell 17, 198-212.
- Cho, S. et al. (2010) Cell Cycle 9, 1697-705.
- Shah, N. and Sukumar, S. (2010) Nat Rev Cancer 10, 361-71.
- Mohan, M. et al. (2010) Genes Dev 24, 574-89.
- Nguyen, A.T. and Zhang, Y. (2011) Genes Dev 25, 1345-58.
Limited Uses
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