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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MMP-1 (E8G4F) Rabbit mAb #30075

Filter:
  • WB
  • IF
Western blot analysis of extracts from SCaBER and HT-29 cells using MMP-1 (E8G4F) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative or low expression of MMP-1 protein in HT-29 cells is consistent with the predicted expression pattern.

To Purchase # 30075

Cat. # Size Qty. Price
30075T 20 µl
$153
30075S 100 µl
$339

Product Specifications

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 55
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:4000 - 1:8000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MMP-1 (E8G4F) Rabbit mAb recognizes endogenous levels of total MMP-1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human MMP-1 protein.

Background

The matrix metalloproteinase (MMP) family of proteases is a group of zinc-dependent enzymes that target extracellular proteins, including growth factors, cell surface receptors, adhesion molecules, matrix structural proteins, and other proteases (1,2). Within this family, MMP-1, MMP-8, and MMP-13 have been characterized as a collagenase subfamily of MMPs targeting fibrillar collagen (collagen type I, II, and III) for degradation. In addition to collagen, MMP-1 also has activity toward a broad array of other ECM proteins, such as fibronectin, gelatin, aggrecan (etc.), as well as growth factors, chemokines, and cytokines (3). MMP-1 is widely involved in tissue remodeling during wound healing, tumor growth, invasion and metastasis, and arthritis (4-6).

Pathways

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For Research Use Only. Not for Use in Diagnostic Procedures.
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