Render Target: STATIC
Render Timestamp: 2025-01-02T10:45:17.756Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-11-18 18:28:10.443
Product last modified at: 2025-01-01T09:06:29.755Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

MO25α/CAB39 (C49D8) Rabbit mAb #2716

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 39
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MO25α/CAB39 (C49D8) Rabbit mAb detects endogenous levels of total MO25α/CAB39 and MO25β/CAB39L proteins.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human MO25α/CAB39.

    Background

    AMPK (AMP-activated protein kinase) is a key cellular component that regulates energy homeostasis (1,2). When the AMP/ATP ratio is increased due to energy depletion, AMPK is phosphorylated at its α1 and α2 catalytic subunits and activated (3). Studies showed that the tumor suppressor LKB1 phosphorylates and activates AMPK in mammals (4,5). MO25α (mouse protein 25α), also called CAB39 (calcium binding protein 39), is a component of the LKB1 complex in vivo (5,6). MO25α/CAB39 does not directly interact with LKB1; instead, it is physically associated with STRADα (6). Together MO25α/CAB39 and STRADα help anchor LKB1 in the cytoplasm (6). MO25α/CAB39 stablizes the LKB1-STRADα complex and further increases the catalytic activity of LKB1 (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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