Render Target: STATIC
Render Timestamp: 2024-12-20T11:40:01.622Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-24 09:09:26.152
Product last modified at: 2024-11-22T15:30:10.808Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody (Isotype Control)
PDP - Template ID: *******4b30f73
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Mouse (E1D5H) mAb IgG3 Isotype Control #37988

Filter:
  • IP
  • IHC
  • IF
  • F
  • ChIP

    Supporting Data

    Concentration 2.5 mg/ml
    SENSITIVITY Endogenous
    Source/Isotype Mouse IgG3
    Application Key:
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    • ChIP-Chromatin Immunoprecipitation 

    Product Information

    Product Usage Information

    Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Mouse (E1D5H) mAb IgG3 Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG3 monoclonal antibodies.

    Background

    Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
      Alexa Fluor is a registered trademark of Life Technologies Corporation.
      All other trademarks are the property of their respective owners. Visit our Trademark Information page.