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Mouse Fc Receptor Blocking Solution (CD16/CD32) Rat mAb

Mouse Fc Receptor Blocking Solution (CD16/CD32) Rat mAb #88280

Supporting Data

REACTIVITY
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rat IgG2b

Product Information

Product Usage Information

To block the interaction of IgG with mouse Fc receptors, add 1.0 μg of antibody per million cells in 100 µl of buffer. Incubate 10 mins at 4ºC or room temperature as desired. A wash step is not needed prior to adding antibodies of interest. The optimal blocking concentration may be assay dependent and may require titration.

Storage

Supplied in 10 mM NaH₂PO₄, 150 mM NaCl, 0.09% NaN₃, pH7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Do not freeze.

Specificity / Sensitivity

Mouse Fc Receptor Blocking Solution (CD16/CD32) Rat mAb binds to all isoforms of mouse CD16 and CD32, preventing interaction with the IgG Fc domain.

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography.

Background

The 2.4G2 clone is widely used as a blocking reagent to prevent interactions between mouse Fc receptors and IgG. By binding to CD32 (FcgammaRII) and CD16 (FcgammaRIII), the 2.4G2 clone prevents the Fc receptor proteins from being able to interact with the Fc domain of antibodies subsequently added for analysis. Performing an Fc blocking step thus reduces background and false positive events in flow cytometry assays on live cells.

Database Links

UniProt ID: P08101-2 , P08508

Entrez-Gene Id: 14130 , 14131

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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