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Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-16 17:30:20.804
Product last modified at: 2024-12-17T19:05:21.318Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit

Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit #97624

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit provides an economical means of characterizing the extent of M1 and M2 macrophages in formalin-fixed, paraffin-embedded tissue samples.

    Specificity / Sensitivity

    Each antibody in the Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit detects endogenous levels of its target human protein. Arginase-1 (D4E3M™) XP® Rabbit mAb does not cross-react with arginase-2. CD206/MRC1 (E6T5J) XP® Rabbit mAb recognizes mouse CD206/MRC1 protein and is also reactive with human CD206/MRC1; however, this antibody is not suggested for immunohistochemical analysis of human tissues. Instead, CD206/MRC1 (E2L9N) Rabbit mAb #91992 is recommended for IHC analysis of human tissue samples. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry using CD68 (E3O7V) Rabbit mAb.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of mouse CD206/MRC1 protein and surrounding Ala1153 of mouse CD11c protein, Val47 of human arginase-1 protein, and Ala139 of human Iba1/AIF-1 protein, or with recombinant proteins specific to mouse F4/80, CD86, and CD68.

    Background

    Macrophages are myeloid cells of the innate immune system that are found in all human tissues in the body and exhibit anatomical and functional diversity. These heterogenous cells are derived from monocyte precursors in the blood that infiltrate into the tissues and differentiate in the presence of cytokines and growth factors. A spectrum of different macrophage phenotypes, or polarizations, have been described based on their secretory profiles, gene expression, and functions. Macrophages have great plasticity and can switch from one phenotype to another under different conditions. At the opposite extremes of this spectrum are so called M1, or classically activated phenotype, and M2 or alternatively activated phenotype. M1 macrophages are generally inflammatory and anti-tumor, while M2 macrophages, commonly referred to as tumor-associated macrophages (TAMs), are generally anti-inflammatory and pro-tumor. Relative contents of M1 and M2 macrophages in the tumor microenvironment may have prognostic values. Modulating macrophage polarization is actively pursued as a therapeutic intervention for many different diseases (1-6).
    In mice, F4/80, CD68, and Iba1/AIF-1 are considered general markers for macrophages. CD86, CD11c, and others have been used as markers for M1 macrophages, while CD206, Arginase-1, and others have been used as markers for M2 macrophages (7-10).

    Database Links

    UniProt ID: P55008 , P31996 , Q61549 , P42082 , P05089 , Q61830 , Q9QXH4


    Entrez-Gene Id: 199 , 12514 , 13733 , 12524 , 383 , 17533 , 16411


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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