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Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-27 17:13:10.327
Product last modified at: 2024-10-15T18:45:10.285Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Mouse Reactive PANoptosis Antibody Sampler Kit

Mouse Reactive PANoptosis Antibody Sampler Kit #70934

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Mouse Reactive PANoptosis Antibody Sampler Kit provides an economical means of detecting the activation of PANoptosis in mouse samples. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Mouse Reactive PANoptosis Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-MLKL (Ser345) (D6E3G) Rabbit mAb recognizes endogenous levels of mouse MLKL protein only when phosphorylated at Ser345. Weak, non-specific nuclear staining has been observed by immunofluorescence (IF-IC). Caspase-3 (D3R6Y) Rabbit mAb recognizes endogenous levels of total caspase-3 protein. This antibody detects full-length caspase-3 as well as the large subunit (p20) of caspase-3 resulting from cleavage during apoptosis. Phospho-RIP3 (Thr231/Ser232) (E7S1R) Rabbit mAb recognizes endogenous levels of RIP3 protein only when phosphorylated at Thr231/Ser232. This antibody may not recognize RIP3 when only singly phosphorylated at Thr231 or Ser232. Gasdermin D (E9S1X) Rabbit mAb recognizes endogenous levels of total Gasdermin D protein. This antibody recognizes the 30 kDa amino-terminal fragment produced during pyroptosis by caspase-1. Cleaved Gasdermin D (Asp276) (E3E3P) Rabbit mAb recognizes endogenous levels of the amino fragment of mouse Gasdermin D protein only when cleaved at Asp276. IL-1β (D3H1Z) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total mouse IL-1β protein. This antibody can detect 500 pg of mature recombinant mouse IL-1β. Cleaved-IL-1β (Asp117) (E7V2A) Rabbit mAb (Mouse Specific) recognizes endogenous levels of mouse IL-1β protein only when cleaved at Asp117.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse MLKL, or synthetic peptides corresponding to residues surrounding Leu60 of mouse Gasdermin D, Asp276 of mouse Gasdermin D, His124 of mouse IL-1β, Asp117 of mouse IL-1β, Val370 of mouse RIP3, or synthetic phosphopeptides corresponding to residues surrounding Ser345 of mouse MLKL and Thr231/Ser232 of mouse RIP3, or recombinant protein specific to the p20 subunit of human caspase-3 protein.

    Background

    Programmed cell death (PCD) plays important roles in organismal development and immune responses. There are three major PCD pathways: apoptosis, pyroptosis, and necroptosis. Apoptosis is a non-inflammatory cell death and is characterized by a series of proteolytic cleavage, beginning with the initiator caspases (caspases-8/9), then the executioner caspases (caspases-3/6/7), followed by cleavage of substrate proteins to drive apoptotic cell death (1,2). During pyroptosis, caspase-1 is proteolytically activated through a protein complex called inflammasome, then the activated caspase-1 can cleave Gasdermin D (GSDMD), IL-1β, and IL-18. The freed GSDMD N-terminal domains from the cleavage form pores in the plasma membrane to drive pyroptotic cell lysis and release of the cleaved and matured IL-1β and IL-18, as well as damage-associated molecular patterns (DAMPs) (3,4). The key steps in necroptosis include the receptor-interacting protein kinase 3 (RIPK3)-dependent phosphorylation of mixed lineage kinase domain-like protein (MLKL), translocation of phosphorylated MLKL to plasma membrane, and disruption of plasma membrane integrity (5,6). In contrast to the non-inflammatory nature of apoptosis, both pyroptosis and necroptosis are proinflammatory (7). While early studies of these PCD pathways focused on their distinct individual features and underlying mechanisms, recent findings point to crosstalk and redundancies among these processes under certain conditions, where the three pathways are activated, not independently of each other, and compensatory responses occur when one pathway is blocked. This new form of PCD with key features of pyroptosis, apoptosis, and/or necroptosis has been termed PANoptosis (8,9). PANoptosis is a coordinated cell death pathway driven by a cytoplasmic protein complex named the PANoptosome, whose components provide scaffold and catalytic functions to engage pyroptosis, apoptosis, and/or necroptosis (10,11).

    Database Links

    UniProt ID: Q9QZL0 , P10749 , P42574 , Q9D2Y4 , Q9D8T2


    Entrez-Gene Id: 56532 , 16176 , 836 , 74568 , 69146


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