Render Target: STATIC
Render Timestamp: 2024-12-13T11:47:28.593Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:59:05.926
Product last modified at: 2024-11-19T19:45:09.867Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MTA3 (E3X2E) Rabbit mAb #37489

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 62, 66
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Chromatin IP 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MTA3 (E3X2E) Rabbit mAb recognizes endogenous levels of total MTA3 protein. This antibody does not recognize MTA1 or MTA2 protein. This antibody detects a 200 kDa protein of unknown origin.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val438 of human MTA3 protein.

    Background

    Metastasis associated gene 3 (MTA3) is a cell-type specific subunit of the NuRD deacetylase complex. Much like MTA1 and MTA2, MTA3 does not associate with other family members, forming a unique NuRD complex. MTA3 has been shown to be estrogen-dependent and a key regulator of Snail and E-cadherin in breast cancer (1). B lymphocyte differentiation has been shown to be dependent on the ability of BCL6 to use MTA3 as a cofactor (2). MTA3 has also been shown to repress the Wnt4 pathway (3). MTA3 activity has been described in many different cancer types, including breast, non-small cell lung cancer, and B-cell lymphomas (1,4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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