Render Target: STATIC
Render Timestamp: 2024-11-22T11:27:22.259Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-11-18 16:03:16.013
Product last modified at: 2024-11-19T09:00:36.156Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MTF2 (F2X1A) Rabbit mAb #46454

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 50, 55, 70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Chromatin IP 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MTF2 (F2X1A) Rabbit mAb recognizes endogenous levels of total MTF2 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly521 of human MTF2 protein.

    Background

    Metal regulatory transcription factor 2 (MTF2), also referred to as polycomb-like 2 (PCL2), is a catalytically inactive member of the PCL protein family (1). MTF2 binds to the metal response element of the mouse metallothionein promoter (2,3) and has also been shown to regulate the PRC2 activity at specific developmental genes in embryonic stem cells (4,5). It was also demonstrated that MTF2 promotes the proliferation and invasion of hepatocellular carcinoma cells (6) and that the MTF2–MDM2 axis can be targeted to sensitize refractory acute myeloid leukemia (AML) to chemotherapy (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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