Render Target: STATIC
Render Timestamp: 2024-12-20T10:58:08.914Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:19.646
Product last modified at: 2024-12-17T18:55:46.515Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MTX1 (E5L8N) Rabbit mAb #47961

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MTX1 (E5L8N) Rabbit mAb recognizes endogenous levels of total MTX1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro400 of human MTX1 protein.

    Background

    Metaxin-1 (MTX1) and Metaxin-2 (MTX2) are part of the sorting and assembly machinery (SAM) that has been associated with import of β-barrel proteins through the mitochondrial outer membrane (1-3). MTX1 and MTX2 can also function in mitochondrial trafficking in neurons (4). Studies of cells with a loss of MTX1 have shown that MTX1 is required for some forms of cell death, including TNF-α-induced cell death (5). Subsequent studies found that MTX1 and MTX2 can regulate TNF-α mediated apoptosis through association with the apoptotic effector protein Bak, and that phosphorylation of MTX1 controls Bak activation (6,7). MTX1 has also been shown to be targeted for basal piecemeal mitophagy in an LC3C- and p62/SQSMT1-dependent manner (8). MTX1 was identified as a regulator of sorafenib resistance in hepatocellular carcinoma by augmenting autophagy (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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