MUC1-C (D5K9I) XP® Rabbit mAb #16564
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #50160.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
MUC1-C is the carboxy-terminal transmembrane subunit of MUC1 resulting from proteolytic cleavage of the full length protein. MUC1-N is the amino-terminal subunit, which can be tethered to MUC1-C, or released from the plasma membrane. MUC1-C interacts with receptor tyrosine kinases, β-catenin and other signaling proteins, and is thought to induce activation of MAPK, Akt and Wnt pathways. Due to its signaling functions and expression in human cancer, MUC1-C is a potential therapeutic target (reviewed in 9).
- Baldus, S.E. et al. (2004) Crit Rev Clin Lab Sci 41, 189-231.
- Schroeder, J.A. et al. (2001) J Biol Chem 276, 13057-64.
- Li, Y. et al. (1998) Mol Cell Biol 18, 7216-24.
- Li, Y. et al. (2001) J Biol Chem 276, 6061-4.
- Ren, J. et al. (2002) J Biol Chem 277, 17616-22.
- Schroeder, J.A. et al. (2004) Oncogene 23, 5739-47.
- Raina, D. et al. (2004) J Biol Chem 279, 20607-12.
- Wei, X. et al. (2005) Cancer Cell 7, 167-78.
- Kufe, D.W. (2013) Oncogene 32, 1073-81.
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